Study of relationship between nitric oxide and TH1/TH2 cytokines in patients with recurrent spontaneous abortion

Objective: To study the role and relationship of nitric oxide with TH1/TH2 cytokines in patient with recurrent spontaneous abortion (RSA). Methods: Serum and placenta NO were measured by nitrate reductase in 101 RSA and 30 nonpregnant women, 97 normal pregnant women, and 30 artificial abortion served as the control group. At the sametime ,serum TNF, IL-6,IL-10 were determined by enzyme linked immunosorbant assay (ELISA). Results: TNF, IL-6,NO in RSA groups were obviously higher than those in the normal control group (P0.05),IL-10 in RSA was markedly lower than that in the control group (P0.01).The concentration of NO in RSA was positively correlated with TNF (r=0.72,P0.05), and negatively correlated with IL-10(r=-0.52,P0.05). Conclusion: Normal level of NO maintain physiological changes in normal pregnant women. It may cause recurrent spontaneous abortion if NO content is very high. Th1/Th2 cytokines are related to recurrent spontaneous abortion. Two types of cytokines are regulated by various facts, of which NO plays an important role in regulating the balance of Th1/Th2 cytokines.