Optimization technology of enzymatic hydrolysis to crucian carp protein of Africa

Taking the degree of hydrolysis(DH) and sensory evaluation as indexes,the result was obtained as follows by single factor test and orthogonal experiment.The results showed that papain and alkaline proteinase was more effective to hydrolyze protein of crucian carp.The optimal hydrolysis condition by papain was gotten pH6.0,solid-liquid ratio of 1∶5,enzyme concentration of 5 % at 60 ℃ for 4 hours.The optimal hydrolysis condition by alkaline proteinase was amount of enzyme 5 %,solid-liquid ratio 1∶3,pH8.0,at 65 ℃ for 4.5 h.The optimum conditions with two enzymes hydrolysis was as: alkaline proteinase and papain were added simultaneously,the ratio of alkaline proteinase / papain was 1∶2,enzyme amount 5 %,solid-liquid ratio 1∶5,pH8.0,at 65 ℃ for 4.0 h.The degree of hydrolysis with two enzymes was over 31.95 %,hydrolysate produced was luster-transparent,better flavour,no fishy smell or bitterness basically.