Study on the hydrolysis of silver carp protein by different enzyme

This paper studied enzyme hydrolysis of Silver carp.Enzymatic hydrolysis conditions of neutral protease,alcalase,trypsin,papain,animal protease and flavorzyme were studied.Single factor tests were adopted to obtain conditional parameters and optimum scope for application.Then through the hydrolysis degree selected the best conditions.Then through the hydrolysis degree and the comprehensive flavor hydrolyzed selected the best conditions and best enzymatic hydrolysis enzyme.The research contents and results in details showed as follows: flavorzyme,animal protease,papain were rather appropriate.Hydrolysis degree and comprehensive score for hydrolyzed were 16.97% and 8.4,23.38% and 7.0,26.34% and 5.6;Optimization hydrolysis craft condition of three enzyme was as following: input amount of enzyme was 0.7%(W∶W),temperature was 55 ℃,pH value were 8.0,8.0,7.0,time of hydrolysis was 4 h,solid-liquid ratio was 1∶5(W∶V).