The effect of gemcitabine cell proliferation and apoptosis of of human hepatocellular carcinoma HepG2 cell

Objective:To investigate the effect of gemcitabine(GEM) on cell proliferation and apoptosis of on hepatocellular carcinoma HepG2 cell line and the molecular mechanism.Methods:The cell proliferation inhibition of HepG2 cells by GEM was observed by MTT assay.The cell cycle and apoptosis of HepG2 cells exposed to GEM was analyzed by flow cytometry.The expressions of survivin and Bcl-2 after GEM treatment were examined by immunocytochemical and Western blot method.Results:Within 0.3715-5 mg/L,GEM obviously inhibited the proliferation of HepG2 cells in a time-and dose-dependent manner.0.3715 mg/L GEM caused HepG2 cells to undergo G0/G1 arrest and induced apoptosis.Immunohistochemical method and Western blot method showed that the expression of Survivin and Bcl-2 were decreased after GEM treatment.Conclusion:GEM can obviously inhibit HepG2 cell proliferation and induce cell apoptosis.The mechanism of this apoptotic effect is closely related to downregulation of expression of survivin and Bcl-2.