The Effect of survivin Gene Silencing Mediated by RNA Interference on Apoptosis and Proliferation in Hepatocellular Carcinoma Cell Line HepG2

Objective To observe the effect of the blockage of survivin gene on cell apoptosis and proliferation in hepatocellular carcinoma cell HepG2,and to explore the role of survivin on development in hepatocellular carcinoma.Methods One pair of survivin-siRNA was designed and synthesized through transcription in vitro.survivin-siRNA was transfected into HepG2 cells through liposome.Tansfection efficiency was evaluated by nonspecific small molecular siRNA(Fluorescein conjugate).survivin-mRNA level was detected by RT-PCR.Expression of survivin was investigated by immunohistochemical SABC method.Cell apoptosis and proliferation were analyzed by flow cytometry and MTT,respectively.Re-sults After transfected by survivin-siRNA,the survivin-mRNA level was reduced by 53.8%.The apoptosis and proliferation of HepG2 cells were not significant different from negative and blank control at 48 hours after transfection.Conclusion The blockage of survivin expression in HepG2 cells shows no significant effect on cell apoptosis and proliferation.These findings suggest that survivin might not play a key role in the regulation of apoptosis and proliferation in hepatocellular carcinoma.