Catalytic spectrophotometric determination of trace copper based on oxidation of hydrogen peroxide on amido black 10B

A new catalytic spectrophotometric method for the determination of trace amounts of copper was developed.The method was based on copper catalytic action on discoloring reaction of amido black 10B oxidized by hydrogen peroxide in NaOH medium.The optimum conditions and the kinetic parameters of this reaction were studied.The linear range is 0-30 ng/mL for copper.The detection limit is 3.5×10~(-10) g/mL.The method has been applied to the determination of trace amounts of copper in soybean and hair samples with satisfactory results.