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2011•Yunnan Journal of Traditional Chinese Medicine and Materia MedicaRequires access

Study on Technology for the Separation and Purification of Myrobalan Total Polyphenols by Macroporous Resin

Cong Yan-wei

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Abstract

Through the determination of static adsorption and desorption rate, 7 kinds of macroporous resin were filtered, and then the best operating conditions of the separation and enrichment of the polyphenols myrobalan were confirmed. The results indicated AB-8 macroporous resin had good adsorption and desorption. Optimum adsorption conditions: the concentration of the column material was 1.0 mg/mL, pH 6.0 and the column rate was 2~3BV/h. Optimal elution conditions: the concentration of ethanol was 80% and the elution rate was 2BV/h and the elution volume was 4BV. The content of the myrobalan polyphenol increased twice after the separation and the enrichment.

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What this paper is about

Through the determination of static adsorption and desorption rate, 7 kinds of macroporous resin were filtered, and then the best operating conditions of the separation and enrichment of the polyphenols myrobalan were confirmed. The results indicated AB-8 macroporous resin had good adsorption and desorption. Optimum adsorption conditions: the concentration of the column material was 1.0 mg/mL, pH 6.0 and the column rate was 2~3BV/h. Optimal elution conditions: the concentration of ethanol was 80% and the elution rate was 2BV/h and the elution volume was 4BV. The content of the myrobalan polyphenol increased twice after the separation and the enrichment.

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Available abstract

Through the determination of static adsorption and desorption rate, 7 kinds of macroporous resin were filtered, and then the best operating conditions of the separation and enrichment of the polyphenols myrobalan were confirmed. The results indicated AB-8 macroporous resin had good adsorption and desorption. Optimum adsorption conditions: the concentration of the column material was 1.0 mg/mL, pH 6.0 and the column rate was 2~3BV/h. Optimal elution conditions: the concentration of ethanol was 80% and the elution rate was 2BV/h and the elution volume was 4BV. The content of the myrobalan polyphenol increased twice after the separation and the enrichment.

Key concepts: Elution, Adsorption, Chromatography, Chemistry, Desorption, Polyphenol, Ethanol, Recovery rate

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