Effects of local application of insulin plus recombinant human epidermal growth factor on wound healing in rats with II degree deep scald

Objective To investigate the effects of local application of small dose insulin plus recombinant human epidermal growth factor(rhEGF) on wound healing in rats with Ⅱ degree deep scald.Methods 96 SD rat models with Ⅱ degree deep scald were randomly assigned to receive local injection of normal saline(group A),0.1 U insulin(group B), 600 IU rhEGF(group C), and 0.1 U insulin plus 600 IU rhEGF(group D).The wound healing period, the wound healing rates, the morphological changes of wound tissue, and the blood glucose in the 4 groups were recorded.Results As compared with group A, B, and C, the wound healing period was significantly decreased(P 0.05), the wound healing rate in day 7, 11, 14,17, and 21 was obviously increased(P 0.05), the contraction and epithelization of wound were more obvious, and the numbers of fibroblasts and capillaries were increased in group D.The blood glucose in group A and C were stable in 7 h after scald, while that in group B and D were decreased obviously 1 h after injection(P 0.05),but gradually returned to normal 5 h after injection.Conclusion Local application of insulin plus rhEGF can accelerate the wound healing in scalded rats.