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2010•Journal of Jiangxi University of Traditional Chinese MedicineRequires access

Fingerprints of Isatidis Radix by HPLC

Yuesheng Wang

Open publisher page 3 citations

Abstract

Objective:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Isatidis Radix.Methods:HPLC method was set up,using phenomenex luna C18 column(4.6 mm×250 mm,5 μm) with gradient elution,the mobile phase was comprised of acetonitrile and formic acid(0.1%),the UV detection wavelength was at 254 nm,with a flow rate of 0.5ml/min.Results:The HPLC fingerprints was set up.Conclusion:The quality of Isatidis Radix can be controlled effectively by HPLC fingerprints.

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What this paper is about

Objective:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Isatidis Radix.Methods:HPLC method was set up,using phenomenex luna C18 column(4.6 mm×250 mm,5 μm) with gradient elution,the mobile phase was comprised of acetonitrile and formic acid(0.1%),the UV detection wavelength was at 254 nm,with a flow rate of 0.5ml/min.Results:The HPLC fingerprints was set up.Conclusion:The quality of Isatidis Radix can be controlled effectively by HPLC fingerprints.

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Available abstract

Objective:To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Isatidis Radix.Methods:HPLC method was set up,using phenomenex luna C18 column(4.6 mm×250 mm,5 μm) with gradient elution,the mobile phase was comprised of acetonitrile and formic acid(0.1%),the UV detection wavelength was at 254 nm,with a flow rate of 0.5ml/min.Results:The HPLC fingerprints was set up.Conclusion:The quality of Isatidis Radix can be controlled effectively by HPLC fingerprints.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Formic acid, Radix (gastropod), Gradient elution, Acetonitrile, Biology

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