2005•Yaoxue jinzhanRequires access

Study on the Fingerprints of Radix Scrophulariae by HPLC/UV

Yu Jing, Zunjian Zhang, Chunhua Yang

Abstract

Objective:To develop a method for studying the HPLC/UV fingerprints of Radix scrophulariae.Methods:Separation was performed on Agilent ZORBAX SB-C_(18)(250 mm×4.6 mm,5 μm)analytical column.The mobile phase consisted of 0.1% formic acid and methanol as gradient elute.The flow rate was 1 mL/min.Results:Perfect fingerprints were obtained which can be used for the valuation of Radix scrophulariae.Conclusion:The method can be used for the quality control of Radix scrophulariae.

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Key concepts: Radix (gastropod), Formic acid, High-performance liquid chromatography, Chromatography, Gradient elution, Materials science, Chemistry, Botany