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Research of Enzymatic Hydrolysis of Tilapia Protein and Deodorization of Hydrolysates

Jian Wu

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Abstract

Alcalase was used to hydrolysis tilapia protein and the hydrolysis parameters were optimized.D113 cation exchange resin was used to deodorization.Results showed that freezing pretreatment increase the degree of hydrolysis(DH)and a maximum DH of 18.23 % was obtained by hydrolysis for 3.0 h using alcalase at 60.0 ℃,pH8.0,enzyme dosage 3 000 U/g(basis of substrate protein),and liquid/solid ratio 3:1(g/g).A clarified product with low fishiness,light golden color was obtained after treated with D113 resin.

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What this paper is about

Alcalase was used to hydrolysis tilapia protein and the hydrolysis parameters were optimized.D113 cation exchange resin was used to deodorization.Results showed that freezing pretreatment increase the degree of hydrolysis(DH)and a maximum DH of 18.23 % was obtained by hydrolysis for 3.0 h using alcalase at 60.0 ℃,pH8.0,enzyme dosage 3 000 U/g(basis of substrate protein),and liquid/solid ratio 3:1(g/g).A clarified product with low fishiness,light golden color was obtained after treated with D113 resin.

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Available abstract

Alcalase was used to hydrolysis tilapia protein and the hydrolysis parameters were optimized.D113 cation exchange resin was used to deodorization.Results showed that freezing pretreatment increase the degree of hydrolysis(DH)and a maximum DH of 18.23 % was obtained by hydrolysis for 3.0 h using alcalase at 60.0 ℃,pH8.0,enzyme dosage 3 000 U/g(basis of substrate protein),and liquid/solid ratio 3:1(g/g).A clarified product with low fishiness,light golden color was obtained after treated with D113 resin.

Key concepts: Hydrolysis, Hydrolysate, Chemistry, Enzymatic hydrolysis, Tilapia, Chromatography, Substrate (aquarium), Enzyme

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