Comparison of Insulin-resistance Rat Models Induced by High-fat and High-carbohydrate Diets

Objective To measure the biochemical parameters in blood of Insulin-resistance rat model induced by high-fat and high-carbohydrate diets.Methods Male SD rats were fed on standard laboratory chow,high-fat diet or high-carbohydrate diet respectively for 6 weeks.Serum glucose(GLU),insulin,triglyceride(TG),total cholesterol(TCH) and HDL-cholesterol were measured at fortnightly interval.The insulin sensitivity was estimated by ISI index,ISI=ln1/(FPG×FINS).Results Compared with control after 6 weeks,ISI index significantly decreased in both high-fat and high-carbohydrate groups(P0.05),and serum insulin was significantly higher and HDL-cholesterol was significant lower in high-fat group(P0.05).Serum GLU and insulin in high-carbohydrate group were higher than those in other groups.However there were mild difference in TG、GLU and body weight among the three groups.Conclusion Both high-fat and high-carbohydrate diets can induce insulin-resistant in rats.High-fat feeding results in significantly high serum insulin and low HDL-cholesterol,but high-carbohydrate treatment leads to high serum GLU and insulin.