The effect of RAD001 inhibiting cell growth of gastric cancer cells SGC7901 and its molecular mechanisms

Objective: To investigate the role of mTOR specific inhibitor RAD001 in inhibiting gastric cancer cell SGC7901 prolif-eration and the possible mechanism of survivin in the inhibition.Methods:SGC7901 cells were cultivated,treated with RAD001 alone or in combination with cisplatin.MTT,flow cytometry,Immunocytochemistry and Western-blot array were used to investigate the cell proliferation,apoptosis and the expression of survivin in cells.Results:After treated with RAD001 alone or in combination with cisplatin,the cell proliferation were inhibited in a dose-dependment manner,and the inhibition rates increased with the treat-ment time extending.High concentration of RAD001 could induce apoptosis of SGC7901 cells.The expression of survivin in SGC7901 cells decreased after treatment.Conclusion:RAD001 could inhibit the cell proliferation of SGC7901 and induce the apoptosis through down-regulating the expression of survivin.