Effect of Aitongke on proliferation,apoptosis of human gastric cancer cells SGC-7901 and its mechanisms

Objective To investigate the anti-cancer mechanisms of aitongke,its impact on proliferation,apoptosis and its underlying mechanisms in human gastric cancer cells SGC-7901.Methods SGC-7901 cells were treated by aitongke of different concentrations,the proliferating rate,the apoptosis rate,cell cycle and retinoblastoma(Rb) gene mRNA level change of SGC-7901 cells were evaluated by CCK method,flow cytometry analysis and RT-PCR method respectively.Results Aitongke(2～50mg/L) could effectively inhibited SGC-7901 cell proliferation and induced cell apoptosis,both with a time-dose dependent manner.Cells accumulated in the gap 1(G1) phases,while reduced in the gap 2 and mitosis(G2/M) phases of the cell cycle,and the apoptosis peak significantly occurred.Aitongke could upregulate the expression of Rb gene,and with a dose dependent manner.Conclusion Aitongke has a potential role on anti-gastric cancer by inhibiting proliferation of SGC-7901 cell,as well as inducing cell apoptosis.And the apoptosis-inducing mechanism is mediated through the G1 blocking of cell cycle in SGC-7901 cells involved with the up-regulating expression of Rb gene.