Dynamic observation and analysis of antibiotic resistance of ESBLs-producing Escherichia coli isolate during last six years

Objective To investigate and analyse the isolation rate and antibiotic resistance rate of extended spectrum beta-lactamases(ESBLs)-producing Escherichia coli from January 2001 to December 2006 in our hospital for correct and reasonable use of antibiotics.Methods ESBLs were detected by the method recommended by National Committee for Clinical Laboratory Standards(NCCLS)or Clinical and Laboratory Standards Institute(CLSI).The antibiotic susceptibility was tested by the Kirby-Bauer method and the data were analyzed by the software of WHONET5.3.The antibiotic susceptibility test data in either ESBLs-producing or non-producing strains was analyzed comparatively.Results Of 1 053 strains of Escherichia coli,445 strains were ESBLs-producing Escherichia coli.The positive rate was 42.3%,and the elevated mode by years was shown.The antibiotic susceptibility tests in vitro showed that imipenem,cefoxitin,amikacin,piperacillin-tazobactam and cafoperazone-sulbactam were effective to ESBLs-producing Escherichia coli.The ESBLs-producing Escherichia coli displayed the multi-antibiotic resistance,and the drug resistance rate in ESBLs-producing strains was higher than that in non-producing strains.Conclusions The positive rate of ESBLs-producing Escherichia coli increased year by year in our hospital during last 6 years.Imipenem,cefoxitin,amikacin,piperacillin-tazobactam and cafoperazone-sulbactam are suitable for clinical treatment of infection caused by ESBLs-producing Escherichia coli strains.