THE EFFECTS OF TRIPTOLIDE ON ANTIGEN EXPRESSION OF PROLIFERATING CELL NUCLEAR FROM BOVINE LENS EPITHELIAL CELLS

Objective: To determine the effects and mechanisms of the Triptolide on antigen expression of proliferation cell nuclear from bovine lens epithelial cells (BLECs) in vitro. Methods: The fourth generation of incubated BLECs were cultured for 24 hours with the recombinant human Epithelial Growth Factor (rhEGF)of 50 μg/L and Triptolide was added in different concentrations(40,20,10 μg/L)for 6～72 hours 6,12, 24, 48 and 72 h respectively. The expression rates of proliferating cell nuclear antigen (PCNA) were measured with FCM(Flow Cytometry) in order to observe and determine the inhibitory effects of Triptolide on the proliferation of BLECs. Results: With the different concentrations of Triptolide (40～10 μg/L)- the expressions of PCNA 6～72 h showed down regulation following the higher concentrations and the longer time. The expression of PCNA in 6,12,24,48,72 h at the concentration of 10 μg/L group were (76.46±0.8),(70.32±1.6),(61.96±2.1),(54.98±2.0), (42.15±1.6) respectively comparing with the proliferation groups at the same time- the differences were significant. P0.05. There were (68.58±3.2),(55.34±2.6),(49.59±1.6),(36.61±0.5),(30.00±1.1) in the same time interval at the concentration of 20 μg/L group; and(60.96±1.2),(46.45±1.7),(32.10±0.5),(27.15±1.1),(19.16±1.5) at the concentration of 40 μg/L group respectively. Comparing with other proliferation groups of the same time interval- there were great significant differences P0.01-which showed both time and dose dependent relations. Conclusion: Triptolide decreasesexpression rate of PCNA- changes the cell cycle and inhibits effectively the proliferation of BLECsin vitro.