Effects of triptolide on proliferative inhibition and lntracellular cGMP in cultured bovine Lens epithelial cells

Objective: To investigate the effects of Triptolide on the proliferative inhibition and intracellular cGMP in cultured bovine-lens epithelial cells(BLECs) in vitro.Methods:The fourth generation of BLECs were collected and cultured into 3 groups : the first group with DMEM,the second with DMEM +50 μg/L rhEGF and the third with 50 μg/L rhEGF+20 μg/L Tri +DMEM. The effects of Triptolide on the proliferation BLECs were measured with MTT colorimetry and the intracellular cGMP was measured by radioimmunoassay.Results: Triptolide at 20 μg/L concentration showed significant inhibitory effect on rhEGF-induced proliferation of BLECs at 6 h,12 h,24 h,48 h and 72 h time courses respectively,the ratio of proliferative inhibition enhancl distinctively on time dependence.Intracellular cGMP concentration of BLECs cultured with 50 μg/L rhEGF+20 μg/L Tri +DMEM was lower than that of the group cultured with DMEM +50 μg/L rhEGF.Conclusion:Triptolide could effectively inhibit the proliferation of BLECs in vitro.The inhibitory effects of triptolide on the proliferation may accomplished by changing the concentration of the cytosolic cGMP.