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STUDIES ON EXTRACTION OF RESVERATROL FROM POLYGONUM CUSPIDATUM BY MEDIUM-PRESSURE SILICA GEL COLUMN CHROMATOGRAPHY

Wenqiang Su

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Abstract

Extraction,separation,structure identification and content determination of resveratrol from Polygonum cuspidatum Sieb.et Zucc.had been carried out.Orthogonal experiment indicated that optimum extraction condition of resveratrol was:60 ℃,95% ethanol 1∶11(raw material∶ethanol),60 min and 3 times of extraction.Separation experiments with silica gel column under about 3 MPa pressure showed that ethyl acetate:petroleum ether was the ideal (eluant). After recrystallizing, resveratrol content was determined by HPLC to be as high as 99.28%.It can be (demonstrated) that this is a good technique used in resveratrol manufacture.

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What this paper is about

Extraction,separation,structure identification and content determination of resveratrol from Polygonum cuspidatum Sieb.et Zucc.had been carried out.Orthogonal experiment indicated that optimum extraction condition of resveratrol was:60 ℃,95% ethanol 1∶11(raw material∶ethanol),60 min and 3 times of extraction.Separation experiments with silica gel column under about 3 MPa pressure showed that ethyl acetate:petroleum ether was the ideal (eluant). After recrystallizing, resveratrol content was determined by HPLC to be as high as 99.28%.It can be (demonstrated) that this is a good technique used in resveratrol manufacture.

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Available abstract

Extraction,separation,structure identification and content determination of resveratrol from Polygonum cuspidatum Sieb.et Zucc.had been carried out.Orthogonal experiment indicated that optimum extraction condition of resveratrol was:60 ℃,95% ethanol 1∶11(raw material∶ethanol),60 min and 3 times of extraction.Separation experiments with silica gel column under about 3 MPa pressure showed that ethyl acetate:petroleum ether was the ideal (eluant). After recrystallizing, resveratrol content was determined by HPLC to be as high as 99.28%.It can be (demonstrated) that this is a good technique used in resveratrol manufacture.

Key concepts: Chemistry, Chromatography, Silica gel, Resveratrol, Extraction (chemistry), Ethyl acetate, Petroleum ether, High-performance liquid chromatography

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STUDIES ON EXTRACTION OF RESVERATROL FROM POLYGONUM CUSPIDATUM BY MEDIUM-PRESSURE SILICA GEL COLUMN CHROMATOGRAPHY — Research Paper | ScholarLens