Construction of Prokaryotic Expression Vector of Bovine Viral Diarrhea Virus E0 Gene and Its Expression

C24V strains of bovine viral diarrhea virus(BVDV) were used to be inoculated into MDBK cells to extract viral RNA,to amplify BVDV-E0 gene and the fragments obtained were connected with the pET-28a expression vector to be transformed into E.coli BL-21 to screen out the posetive clones.The results showed that identification of pET-28a-E0 prokaryotic expression vector was successfully constructed.The bacteria were collected after IPTG induction by SDS-PAGE and Western-blot identification,protein identification results show that to be expressed in E.coli.