Prokaryotic Expression E2 gene of Bovine Viral Diarrhea Virus Genotype 2 and Reactionogenicity of Recombinant Protein

According to BVDV-2E2gene sequences accessed in GenBank,apair of specific primers was designed to amplify E2 gene of SW isolate.The amplified product was cloned into T vector and sequenced.And then E2gene was subcloned into BL21(DE3)expression vector pET-28afor expression.Recombinant plasmid pET-28a-E2was transformed into E.coli,Analysis of SDS-PAGE and Western blot were conducted for expression products after IPTG induction.SDS-PAGE confirmed that the recombinant fusion protein was expressed with a relative molecular weight of 32ku.Western blot analysis showed that the recombinant protein can specifically be reactive to anti-BVDV-2polyclonal antibody,which confirms that the recombinant E2protein is endowed with good reactionogenicity.The study lays a foundation for developing diagnostic reagents for BVDV-2infection.