Research on preparation of baicalin-phospholipid complex

Objective: To study the optimum conditions for the formation of baicalin complex with phospholipids.Methods: A reasonable criterion of accurate evaluation was established by testing the reaction solvent type,temperature,time,the ratio of baicalin to phospholipid and the concentration of reactants were examined and the orthographic design was used and the best preparation technology was determined.Results: The optimized preparation conditions for baicalin phytosomes were obtained as follows: Solvent was tetrahydrofyran.The ratio of baicalin to phospholipid was 1 to 2.The temperature was 55℃.The reaction time was 1 h.The baicalin concentration was 2.5 mg/mL.Conclusion: The formation of baicalin complex with phospholipid was greatly influenced by the solvent,concentration and temperature;the optimized preparation is feasible and stable.