Determination of Matrine,5-Hydroxymethylfurfural and Related Substances in Injection Containing Matrine and Glucose by HPLC

Objecttive,to study the determination of matrine,5-hydroxymethyfurfural,and related substances in matrine and glucose injection.Methods: the high-performance liquid chromatography(HPLC) method was carried out according to the Chinese Pharmacopoeia,3 batch of samples were determined under the condition: amino-bonded silica as the column,acetonitrile-0.02mol/L NaH2PO4 solution(75∶25) as the mobile phase,the detection wavelength was 210nm and the theoretical plates were more than 5000 calculated by matrine.Results,the calibration curve was linear in the range of 21.966~109.83μg/ml(r = 0.999 9).The mean recovery was 100.43 %,RSD was 2.02 %(n = 9),the determination of 5-Hydroxymethylfurfural and related substances also have good results.Conclusion,this method is sensitive,accurate and reproducible,can be used for quality control of matrine and glucose Injection.