Drug-resistant analysis of 327 strains of gram-negative bacilli

Objective To evaluate the drug resistance of gram-negative bacilli and provide reference for the rational use of antimicrobial agents in clinic.Methods Three hundred and twenty-seven gram-negative bacilli isolated from different clinical samples from January to June in 2004 were identified and the drug sensitive tests were also detected.Results The isolated gram-negative bacilli were Escherichia coli((42.8%)),Pseudomonas aeruginosa((16.8%)),Klebsiella spp.((12.8%)),Acinetobacter spp((8.0%)),Enterobacter spp((6.7%)).71 strains((21.7%)) were ESBLs positive strains,59 of which were Escherichia coli,12 were Klebsiella spp;The resistant rate to piperacillin/tazobactam((1.5%)) is obviously lower than that to piperacillin((89.7%)).All strains had the highest resistant rate to SMZco and ampicillin,which was(94.7%) and(80.9%) respectively,and had the lowest resistant rate to imipenem(8%).The main bacteria isolated from respiratory tract was Pseudomonas aeruginosa(37 strains),except the lower resistant rate to amikacin((8.1%)),the resistant rates to most antimicrobial agents were 40%.Conclusion The isolated gram-negative bacilli had multiply resistance to antimicrobial agents.In the treatment of infection with ESBL-producing strains,compound β-lactamase antimicrobial agents and imipenem should be chosen,third generation cephalosporins should be prudent.