2006•Hubei nongye kexueRequires access

Establishment of the HPLC Fingerprint of Radix Scrophulariae

Bai Zhi-chuan

Abstract

HPLC fingerprint of the methanol-dissolved elements of Radix Scrophulariae collected from different areas provides a new way for scientific evaluation and proficient controlling of Radix Scrophulariae quality.10 samples of Radix Scrophulariae were tested by HPLC,which were from Wulong County,Chongqing City.The HPLC condition was that:Kromasil TM C18 column,washing solution of methanol-1% glacial acetic acid,290 nm wave length,1.0mL·min-1 flow rate,30℃ column temperature.The result was that:the common model of HPLC fingerprint was made and the similarity of Radix Scrophulariae from different areas was compared.The study would be good for the standard planting and quality control of Radix Scrophulariae.

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Key concepts: Radix (gastropod), High-performance liquid chromatography, Fingerprint (computing), Chromatography, Acetic acid, Methanol, Materials science, Computer science