Identification of the Habitat of Radix Scrophulariae with Chromatographic Fingerprinting

Objective To identify the Habital of Radix Scrophulariae with Chromatographic Fingerprinting.Methods Active constituents were extracted from ten batches of sample of Radix Scrophulariae(Scrophularia ningpoensis Hemsl.),which collected from Chongqing Wulong county,and evaluated by HPLC.Chromatographic conditions was Kromasil TM C18 column with methanol-1% glacial acetic acid as mobile phase,the detection wavelength was 290 nm,the flow rate was 1.0 ml·min-1,and column temperature was 30℃.Results Many active constituents that extracted from ten batches of sample of Radix Scrophulariae(Scrophularia ningpoensis Hemsl.),which collected from Chongqing Wulong county,were evaluated by HPLC.The models of standard HPLC Fingerprint of Radix Scrophulariae were established;Taking the cinnamic acid as the reference peak,the relative time and the relative peak area ration of various characteristics peak were calculated,and the sample from Zhejiang province could be distinguished by this model and the relative peak area ratio of the characteristic components.Conclusion This study is good for the standard planting and quality control of Radix scrophulariae.