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2011•Journal of Chengdu University of Traditional Chinese MedicineRequires access

Orthogonal Test for Optimization of Rhizoma Coptidis Extractionin Dahuang-Huanglian Droplet

HE Qian-ling

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Abstract

Objective:To optimize the best extraction process of Rhizoma Coptidis in Dahuang-Huanglian droplet.Method:Using orthogonal design method,to apply extract yield of Rhizoma Coptidis and the extract amount of berberine hydrochloride as observing indexes,to measure the content of berberine hydrochloride with HPLC.Result:The optimum extraction process was as follows,adding 10 times of 60% alcohol into coptis meal,refluxing and extracting for 3 times,one hour every time.Conclusion:This optimized process is simple and Suitable for industrial production.

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What this paper is about

Objective:To optimize the best extraction process of Rhizoma Coptidis in Dahuang-Huanglian droplet.Method:Using orthogonal design method,to apply extract yield of Rhizoma Coptidis and the extract amount of berberine hydrochloride as observing indexes,to measure the content of berberine hydrochloride with HPLC.Result:The optimum extraction process was as follows,adding 10 times of 60% alcohol into coptis meal,refluxing and extracting for 3 times,one hour every time.Conclusion:This optimized process is simple and Suitable for industrial production.

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Available abstract

Objective:To optimize the best extraction process of Rhizoma Coptidis in Dahuang-Huanglian droplet.Method:Using orthogonal design method,to apply extract yield of Rhizoma Coptidis and the extract amount of berberine hydrochloride as observing indexes,to measure the content of berberine hydrochloride with HPLC.Result:The optimum extraction process was as follows,adding 10 times of 60% alcohol into coptis meal,refluxing and extracting for 3 times,one hour every time.Conclusion:This optimized process is simple and Suitable for industrial production.

Key concepts: Berberine hydrochloride, Berberine, Chromatography, Extraction (chemistry), Coptis, Coptis chinensis, Yield (engineering), Chemistry

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