Effects of γ-interferon on proliferation and B7 expression in SPC-A-1 cell line of human lung adenocarcinoma

Objective To study the effects of IFN-γon expression of B7 molecules in human lung adenocarcinoma cell line cultured in vitro.Methods Human lung adenocarcinoma SPC-A-1 cells were incubated in the medium with IFN-γrespectively.Cell proliferation effect was measured by MTT assay;apoptosis was detected with flow cytometry assay;expression of B7-1 and B7-2 mRNA was determined by RT-PCR.Results Compared with those of control group,MTT shows that IFN-γcould reduce the SPC-A-1 cell proliferation.FCM shows the apoptosis rate in the IFN-γ group was significantly higher,but there is no different changes in each concentration group.Compared with the control group,expression of B7-1 and B7-2 significantly increased in the IFN-γgroup,and the expression was not correlated with the concentration of IFN-γin each control group.Conclusion IFN-γcan inhibit cell proliferation and induce apoptosis in SPC-A-1 cell line.IFN-γmarkedly enhance the expression of B7-1 and B7-2 in SPC-A-1 line.The apoptosis may be mediated by up-expression of gene B7-1 and B7-2.