Effects of Z24 on rat liver antioxidation system

Objective To study effects of Z24 on rat liver antioxidation system.Methods Female Wistar rats were intragastric administrated 50,100 and 200 mg/kg Z24 for 5 days,respectively,and the control group were treated with purified water.On the sixth day,rats sacrificed,then malondialdehyde(MDA),reduced glutathione(GSH),Copper and zinc superoxide dismutase(CuZn-SOD),and glutathione peroxidase(GSH-Px) glutathione S-transferase(GST) of liver homogenate were detected,and some blood plasma biochemical indicators,hepatic tissue pathology were detected.Results The levels of alanine aminotransferase(ALT),asparate aminotransferase(AST),alkaline phosphatase(ALP) and total bilirubin(TBI) in 100,200 mg/kg group were higher than those of control group(P0.05)and total bile acid(TBA) level in 200 mg/kg group was lower than the control(P0.05).Hepatocyte vacuolar degeneration and fibroplasias were observed in tissue sections of Z24 treated groups through optical microscope,and pathological changes such as mitochondrial cristae breakage were seen in sections of 200 mg/kg group through electron microscope.These results indicated that Z24 could damage liver at these doses.In liver homogenate,contrasted with the control,activity of CuZn-SOD in 50 mg/kg group was compensated increased,activity of GSH-Px in 100 and 200 mg/kg groups was decreased,and activity of GST in all three Z24 groups was increased,but no higher MDA level and lower GSH level were seen,which indicated rat liver oxidation and antioxidation system still being balanced at toxiferous dose.Conclusion Z24 can suppress GSH-Px activity,but oxidative stress does not play the major role in Z24 induced hepatotoxic effect.