Study on Preparation of Vitamin A-Curcumin Liposome and Its Cytotoxicity

OBJECTIVE To prepare Hepatic stellate cell targeting Vitamin A-curcumin liposome and to investigate its cytotoxicity in vitro.METHODS Reverse phase evaporation was used to prepare VA-curcumin liposome and the entrapment efficiency and the VA binding rate was determined.Entrapment efficiency,VA binging rate and peroxide value were determined to evaluate the stability of liposome at 4 and 25 ℃ for 30 d.MTT assay was used to investigate the cytotoxicity and targeting ability of the liposome in rat hepatic stellate cells HSC-T6 with high expression of retinol binding protein and human esophageal cancer cells A-549 without expression of retinol binding protein.RESULTS The average entrapment efficiency for curcumin was 89.32%,and binding rate for VA was 61.34%.The liposome stored at 4 ℃ was stable.The results of MTT assay showed that IC50 of curcumin,curcumin liposome,and VA-curcumin liposome in HSC-T6 cell were 31.53,14.95 and 8.28 μg·mL-1,respectively.Curcumin liposome and VA-curcumin liposome had same cytotoxic effects in A-549 cell.CONCLUSION The optimized conditions were obtained with high entrapment efficiency and good stability.VA-curcumin liposome could specifically target HSC and promote therapeutic effect of curcumin.