Determination of Chlorogenic Acid Extracted from Flos Lonicerae with High-Speed Homogenization and High Performance Liquid Chromatography

In this paper,three sample preparation for determination of chlorogenic acid in flos lonicerae was studied.Test results showed that the time spent on high-speed homogenization extraction was obviously less than the ultrasonic extraction and the hot reflux extraction.The chlorogenic acid was extracted from samples by high-speed homogenization with 80% methanol solution.The extract was separated on a Waters Xterra RP18 column(3.9mm×150 mm,5 μm) using 1% acetic acid as the mobile phase with gradient elution,the flow rate was 0.8 mL/min.The detection wavelength was 345 nm.Under this condition,the linear of chlorogenic acid was in the range of 5.0—200 μg/mL.The average recoveries of the samples were 118%,and the relative standard deviation was 4.5%(n=6).This method for the extraction and determination of chlorogenic acid in flos lonicerae has good results.