The Significance and Expression of HER2 Gene by Fluorescence in Situ Hybridization in Breast Cancer

Objective:To explore the clinical implications of the expression of HER2 gene by fluorescence in situ hybridization(FISH) in breast cancer.Methods:HER2 gene in 50 cases with female breast cancer was detected by FISH,and the relationship between HER2 gene over-expression and clinical characters was investigated,and the difference by FISH and immunohistochemistry(IHC) was compared.Results:The total coincident rate by FISH and IHC was 88%,HER2 gene over-expression rate was 44% in female breast cancer of our hospital,and not related with tumor size,metastasis and clinical tumor stage(P0.05).There is significance in HER2 gene overexpression between the PR positive and the PR negative group (P0.05) and no significance between ER positive and ER negative group(P0.05).Conclusion:There is no correlation in the over-expression of Her2 gene with tumor size,axillary lymph node metastasis,clinical tumor stages and ER and negative correlation with PR.FISH had good sensitivity and stability in detecting HER2 gene amplification of breast cancer tissue,thus could be conducted to diagnose HER2 gene.