Expression of ZPPS in human breast carcinoma and its relationship with Her-2 gene amplification

Purpose To detect the expression of Zheng's plant protein segment(ZPPS) in breast invasive carcinoma,and to evaluate the relationship of ZPPS's expression in breast invasive carcinoma with clinicopathological parameters and Her-2 gene amplification.Methods The expression of ZPPS was detected in normal breast tissue and breast invasive carcinoma by immunohistochemistry(IHC) in breast carcinoma.The expression of Her-2 gene was detected in breast invasive carcinoma by fluorescence in situ hybridization(FISH).Results The expression of ZPPS was much higher in invasive carcinoma(74.51%) than that in normal breast tissues(34.78%).Twenty of fifty-one(39.22%)cases of breast cancer showed positive for HER-2 gene amplification.The expression of the ZPPS had a significant correlation with Her-2 gene(P0.05).And there was a correlation between the expression of ZPPS and the lymph node metastasis(P0.05).Conclusions ZPPS may play a key role in diagnosing and judging lymph node metastasis in breast invasive carcinoma.The negative relationship between expression of ZPPS with Her-2 gene may suggest Her-2 gene amplification is detection by FISH in the breast cancer with negative ZPPS expression,which to reduce the detection of Her-2 gene and rationally select the cases for molecular targeting therapy of Herceptin in breast cancer.