ScholarLens Back to search
2011•Zhongguo shouyi zazhiRequires access

Establishment of SYBR Green I real-time PCR assay for the detection of porcine parvovirus

Xiaojie Zhang

Open publisher page 1 citations

Abstract

A SYBR GreenⅠreal-time PCR for detecting porcine parvovirus(PPV) was established using primers derived from the published sequences.A plasmid containing pMD18-T-PPV NS1 was constructed and served as a standard template to generate the standard curve.The assay had high specificity and could detect as low as 7 copies of virus DNA.The sensitivity was 100 times greater than that of general PCR.Fifteen clinical samples were detected by this assay,conventional PCR and LAMP at the same time. The results showed that PPV SYBR Green Ⅰ real-time PCR assay is highly sensitive and of low cost.It could be used as an effective tool for the rapid detection of clinical samples.

About this research paper

What this paper is about

A SYBR GreenⅠreal-time PCR for detecting porcine parvovirus(PPV) was established using primers derived from the published sequences.A plasmid containing pMD18-T-PPV NS1 was constructed and served as a standard template to generate the standard curve.The assay had high specificity and could detect as low as 7 copies of virus DNA.The sensitivity was 100 times greater than that of general PCR.Fifteen clinical samples were detected by this assay,conventional PCR and LAMP at the same time. The results showed that PPV SYBR Green Ⅰ real-time PCR assay is highly sensitive and of low cost.It could be used as an effective tool for the rapid detection of clinical samples.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A SYBR GreenⅠreal-time PCR for detecting porcine parvovirus(PPV) was established using primers derived from the published sequences.A plasmid containing pMD18-T-PPV NS1 was constructed and served as a standard template to generate the standard curve.The assay had high specificity and could detect as low as 7 copies of virus DNA.The sensitivity was 100 times greater than that of general PCR.Fifteen clinical samples were detected by this assay,conventional PCR and LAMP at the same time. The results showed that PPV SYBR Green Ⅰ real-time PCR assay is highly sensitive and of low cost.It could be used as an effective tool for the rapid detection of clinical samples.

Key concepts: SYBR Green I, Parvovirus, Real-time polymerase chain reaction, Molecular biology, Plasmid, Virology, Biology, Standard curve

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Establishment of SYBR Green I real-time PCR assay for the detection of porcine parvovirus — Research Paper | ScholarLens