Development of a SYBR Green I Real-time PCR assay for detection of goose parvovirus.

A SYBR Green Ⅰ based Real-time PCR assay was developed for detection of goose parvovirus(GPV)and a recombinant plasmid containing the target gene NS1 was constructed as a standard control.It had a good linear relationship between initial templates numbers and Ctvalues,and the correlation coefficient of the standard curve was 0.998.The assay had a detection limit of 30 copies/μL of initial templates,which was 100 times more sensitive than the conventional PCR.Moreover,the assay had no cross reaction with DPV,CPV,NDV and GPMV,and had a coefficient of variations less than 2% for both intra-and inter-assay.Test on positive clinical samples showed 100% consistency.The high correlativity,sensitivity,specificity and reproducibility of this assay,together with its relatively rapid and simple procedure,indicated that the SYBR GreenⅠreal-time PCR could be used as an effective assay for detection of nucleic acids and quantification of viral loads of GPV.