Optimizing Extraction Process of Total Phenolic Acids in Fructus Aurantii Immaturus by Design-response Surface Methodology

Using ultrasonic wave to extract total phenolic acids in fructus aurantii immaturus,and optimizing the extraction parameters.Ethanol concentration,the ratio of solvent to material and extraction temperature are investigated factors.The extraction rate of the total phenolic acid was the index.Using Box-Behnken statistical method studies the mathematical relationship between the index and 3 factors,mapping the response surface,determing the optimal extraction parameters and validating testing.The optimal ultrasonic extraction process was as follows: the ethanol concentration was 56 %,the ratio of solvent to material was 28 ∶ 1,the temperature was 30 ℃,and extraction times were 2.