Effect of water extracts of ZJF and FZJF on SH-SY5Y cells apoptosis induced by MPP~+

Objective To determine the effect of water extracts of Zuojinfang(ZJF) and Fanzuojinfang(FZJF) on SHSY5 Y cells apoptosis induced by MPP+. Methods SH-SY5 Y cells were cultured in vitro and MPP+ induced SHSY5 Y cells apoptotic model was established and treated with water extracts of ZJF and FZJF. The cell activity was measured by MTT test, the cell apoptosis rate was detected by flow cytometry, and the morphology was observed by Hoechst 33258 staining. Results The apoptosis of SH-SY5 Y cells was induced by 0.5 mmol·L- 1 MPP+ for 48 h, MTT decreased remarkably to(50.87±2.31)%. the apoptosis rate increased to(43.38±1.49)%, and Hochest staining showed cell nuclear condensation, granular fluorescence and other typical features of apoptosis. After the cells were incubated with ZJF/FZJF and 0.5 mmol·L- 1 MPP+, the apoptosis was increased obviously. When the carnitine concentration increased to 2.0 mg·m L- 1, the cell activity increased to(63.16±1.89)% and(75.19±1.97)%, and the percentages of apoptosis decreased to(20.17±1.43)% and(9.41±1.27)% respectively. The difference in comparison with the model group(43.38±1.49)% was statistically significant(P 0. 01). Conclusion Both ZJF(cold) and FZJF(hot) can withstand the apoptosis of SH-SY5 Y cells induced by 0.5 mmol·L- 1 MPP+, where the effect of FZJF is stronger than that of ZJF under the same conditions. With different natures of transitional Chinese medicine, the anti-apoptotic effect is different.