Roles of heat shock protein 10 in apoptosis of SH-SY5Y cells caused by manganese

Objective To explore the relationship between cell viability,apoptosis rate and heat shock protein 10( Hsp10) expression after SH- SY5 Y cells exposure to manganese( Mn2 +). Methods SH-SY5 Y cells were divided into control group( group A,normal saline,0. 9% NaCl) and experimental groups( group B: Mn2 +0. 5 mmol /L,group C: Mn2 +1. 0 mmol /L,group D: Mn2 +2. 0 mmol /L,group E: Mn2 +4. 0 mmol /L). After SH- SY5 Y cells exposed to Mn2 +for 12 h,the cell viability and apoptosis rate in each group was measured by MTT method,and expression of Hsp10 mRNA and protein was detected by RT- PCR and Western blot. Results Compared to control group,the cell viability significantly decreased in each experimental group( P 0. 05),which was 92. 93%,87. 28%,79. 53% and 73. 67%,respectively. The apoptosis rate increased in a dose-dependent manner after exposure to Mn2 +. Similarly,the mRNA and protein expression of Hsp10 presented a notably dose-dependent decrease. Hsp10 mRNA level was 0. 95,0. 84,0. 63,0. 46 folds of the control in groups B,C,D,E and the Hsp10 protein expression was significantly lower than in control group( P 0. 01). Conclusion Manganese can attenuate the cell viability and Hsp10 expression( mRNA and protein) but elevate the rate of apoptosis,which suggest that Hsp10 might play an important role in Mn-induced apoptosis.