Study on Transformation Breeding of Cell Nuclear Sterile Line in Youqingsijiu Brassica campestris

[Objective] The aim of the research was to discuss transformation breeding of cell nuclear sterile line in Youqingsijiu Brassica campestris. [Method] Taking French 45 hot-resistance Chinese cabbage sterile line as sterile source, cell nuclear sterile line and new AB lines type I and temporary maintainer line of Youqingsijiu B. campestris were obtained through transformation breeding of conventional hybrid. [Result] Based on the cross between the fertile lines and the sterile plants in male sterile lines and that in AB lines type I and reciprocal cross between fertile plants in new AB lines type I, the genotypes of fertile lines were all determined as MsfMsf. According to transformation breeding project of male sterile line hybrid, new AB lines type I, male sterile line and temporary maintainer line of Youqingsijiu B. campestris with Chinese cabbage cytoplasm were bred. According to backcross transformation breeding project, transformation breeding of 4 generation male sterile lines with cytoplasm of Chinese cabbage and B. campestris were completed, whose separation proportion of stamen fertility progenies was accorded with backcross transformation breeding project completely. [Conclusion] During the transformation breeding of male sterile line, it would had good effect of transformation breeding to pay attention to select the plants with the same type,