Construction and identification of mouse RPL8 gene recombinant adenovirus vector

Objective To construct the recombinant adenovirus vector encoding mouse RPL8 gene for transfecting dendritic cells (DC). Methods RPL8 gene was cloned by RT-PCR, and after being processed, the gene was transferred into pAdxsi adenovirus vector and was packed into adenovirus gramule. Then the recombinant adenovirus and Ad-EGFP were transfected in mice DC, the expression of the green fluorescencent protein and RPL8 were detected by fluorescencent microscope. Results As confirmed by restriction digestion analysis, an expectant was observed in proper recombinant adenovirus vector encoding RPL8 gene. The viral titer checked by GFP was about 1.6 × 1010 PFU/mL. Conclusions The recombinant adenovirus vector encoding RPL8 gene has been established successfully.