Simultaneous determination of twenty trace sulfonamides residues in honey by ultra-fast liquid chromatography coupled with tandem mass spectrometry

Objective:To develop a fast,efficient,accurate and sensitive method for determination of sulfonamides residues in honey by ultra-fast liquid chromatography tandem mass spectrometry.Methods: Honey samples were extracted using 0.1%(V/V) phosphoric acid,and purified by MCX solid-phase extraction(SPE) cartridges.The separation was carried out on a Shim-pack XR-ODS Ⅱ(100 mm×2.0 mm i.d.,2.2 μm) with acetonitrile and water as mobile phase by gradient elution,which containing 0.1%(V/V) formic acid and 5 mmol/L ammonium acetate.Detection was performed by an ultra-fast liquid chromatography-triple quadrupole tandem mass spectrometry(UFLC-MS/MS) with an electrospray ion(ESI) source in the positive multipile reaction monitoring(MRM) mode.Results: Calibration curves for the sulfonamides except sulfaguanidine and sulfacetamide were linear within the range of 0.25 μg/kg～25.0 μg/kg,and 1.0 μg/kg～25.0 μg/kg for sulfaguanidine and sulfacetamide.The correlation coefficients were more than 0.99,the limits of detection(LODs)were between 0.004 μg/kg～0.25 μg/kg,and the limits of quantification(LOQs)were between 0.01 μg/kg～0.83 μg/kg.The extraction recoveries ranged from 63.3% to 106.9% at three spiked levels of 0.5 μg/kg,2.5 μg/kg,5.0 μg/kg for each sulfonamide,and the RSDs ranged from 1.7% to 12.3%.Conclusion: The developed method can be detected simultaneously 20 sulfonamides within 5.5 min.It is fast and efficient,and which has used satisfactorily for the determination of trace sulfonamides in actual honey samples.