Validation of the dependability of screening models for selective cox-2 inhibitors

Aim To validate the dependability of screening models by culture of rat macrophages in vitro.Methods The PGE2 was detected by using radioimmunoassay in different concentration of serum,stimulating time and concentration of lipopolysaccharide(LPS).The target protein COX-1 and COX-2 were detected by Western blotting in different scrum concentration,stimulating time and concentration of LPS.Results Macrophages produce more PGE2 after stimulated by LPS in concentration of 0.5 μg· L-1 for 6 hours,meanwhile,produced more COX-2 than others.There is not an obvious difference on COX-1 between 0.5% and 10% FCS RPIM-1640,but it decreases visibly after aspirin added to it.Conclusion The screening models can be used for screening selective COX-2 inhibitors of the antiinflammatory drugs.