Optimization of Processing Technology of Diphylleia sinensis Stir-baked with Vinegar by Orthogonal Test

Objective: To optimize processing technology of Diphylleia sinensis stir-baked with vinegar. Method: Taking the content of podophyllotoxin as index,orthogonal test was adopted to optimize processing technology with four factors including moistening time,the amount of vinegar,frying time and temperature. With mobile phase of methanol-0. 1 mol ·L-1potassium dihydrogen phosphate solution(45 ∶ 55) and detection wavelength at 290 nm,the content of podophyllotoxin was determined by HPLC. Result: The best processing technology was as following: moistened 2 h with 30% vinegar,flew 4 min at 150 ℃. The amount of vinegar and frying time had significantly effects on processing technology,the mass fraction of podophyllotoxin was up to 5. 48%. Conclusion: This optimized processing technology was reasonable and feasible. This result could provide a reference for medication safety of D. sinensis in folk.