Effect of curcumin on apoptosis and bFGF expression in lymphoma Raji cells

OBJECTIVE:To detect the effects of curcumin on apoptosis and bFGFs′expression in Raji cells,and provide a theory evidence for therapy of anti-vessel neogenesis.METHODS:After Raji cells were treated with curcumin at different concentrations and different time,the morphological changes of cells were observed by inverted phase contrast microscope,and the cell apoptosis was tested by flow cytometry;the change of bFGF contents in cultured supernatants of Raji cells treated with different concentratins of curcumin were measured by ELISA.RESULTS:The morphological changes of cells were observed by inverted phase contrast microscope,the Raji cells turned into disorder from conglobation,the cell population obviously was decreased and the cell volume shrinked,the refraction weakened,the cell membrance disrupted,cell debris existed.The apoptosis rate of Raji cells was increased as the concentration of curcumin and action time were increased.When curcumin concentrations were 10 and 25 μmol/L,the apoptosis rate of Raji cells were(11.12±1.05)%,(25.07±5.40)% after 24 h,(14.74±0.46)%,(33.50±5.89)% after 48 h and(25.11±1.43)%,(55.98±7.41)% after 72 h,which were higher than that of control group [(1.75±0.28)%,(2.25±0.29)%,(2.77±0.61)%] and there was a significant difference(P0.01).The change of bFGF contents in cultured supernatants of Raji cells treated with different concentratins of curcumin(10,25 μmol/L),in different time(24,48,72 h)were measured by ELISA,the 10 μmol/L curcumin group levels of bFGF were(212.76±20.07),(173.23±14.76) and(107.21±12.37) ρg/mL;the 25 μmol/L curcumin group levels of bFGF were(95.32±11.59),(71.24±10.03) and(36.41±7.58) ρg/mL,significantly lower than that of the control groups(643.41±57.12),(732.26±60.19) and(931.27±81.04) ρg/mL.CONCLUSIONS:Curcumin can promote the Raji cells apoptosis,inhibit bFGF secretion of Raji cells and this function is relied on concentrations and times.These results suggest that curcumin may be anti-vessel neogenesis.