The preliminary study of curcumin's effect on proliferation inhibition of HEC-1-B

Objective To observe the effects of curcumin on cultured human endometrial cancer(HEC-1-B) cell proliferation and cell cycle distribution.Methods HEC-1-B cells cultured in MEM were divided into 5 groups:control and curcuminⅠ,Ⅱ,Ⅲ or Ⅳ group according to different concentrations(10,20,40 or 80) μmol/L.The proliferation of HEC-1-B cells was measured by MTT colorimetric method and western blot in each group at 48 hours after intervention.the induction of cell cycle was detected with flow cytometry.Results MTT results showed that the values of A490nm in curcumin groups were lower than that in the control group at hours 48(P0.01),and the effect of HEC-1-B proliferation gradually decreased with the increasing content of curcumin.The expressions of PCNA decreased in dose-dependence significantly(P0.01) within the range of concentrations(from 10 to 80) μmol/L of Cur,compared with control group.The values of G2/M in curcumin groups were higher than that in the control group at hours 48(P0.01),and the effect for cell cycle gradually increased with the increasing content of curcumin.Conclusion Proliferations in HEC-1-B cells were inhibited,cell cycles were blocked by curcumin in dose-dependence,which could be one of the mechanisms of anti-HEC-1-B of curcumin.