Determination of Lomefloxacin in Milk by Using Second-order Calibration Based on Alternating Penalty Trilinear Decomposition Coupled with Fluorescence Spectroscopy

A simple method for direct determination of lomfloxacin in milk even in the presence of other uncalibrated interferents was described in this paper. This second-order calibration was based on the alternating penalty trilinear decomposition algorithm coupled with fluorescence spectroscopy. When the estimated component number in three-way fluorescent responses was set to 4,the obtained recovery of lomefloxacin in milk was 99.4%±1.3%,and the root-mean-square error of prediction(RMSP) was 2.97 nmol/L. The satisfactory result indicated that the proposed method could be applied to simply quantify lomefloxacin in milk even in the presence of unknown interferences.