Effects of Jiaomu Oil on IL-4 and IFN-γ in bronchoalveolar lavage fluid and NF-κB in lung tissue of asthmatic model in guinea pig

Objective To explore effects of Jiaomu Oil on IL-4 and IFN-γ in bronchoalveolar lavage fluid(BALF) and NF-κB in the lung tissue of asthmatic model in guinea pig and to provide theoretical basis for treatment of bromchial asthma (asthma) using Jiaomu Oil.Methods This experiment included four groups:normal control group,asthmatic model group,dexamethasone treatment group and Jiaomu Oil treatment group,involving 10 guinea pigs in each group.Asthmatic models of guinea pigs were constructed by immunization of intraperitoneal injection with saline ovalbumin(OVA) and challenge of inspiration with nebulized OVA.The levels of IL-4 and IFN-γ in BALF of guinea pigs were determined using enzyme-linked immunosorbent assay (ELISA).The expression of NF-κB in the lung tissue was observed by immunohistochemical staining.Results In the asthmatic model group,the levels of IL-4 and IFN-γ in BALF and PC_(20),(41.36±6.71) ng/L vs (10.58±1.28) ng/L,(21.15±2.75) ng/L vs (73.52±5.23) ng/L, (0.013±0.014) g/L vs (0.168±0.186) g/L,had significant differences compared with those of normal control group,respectively(P0.05).The levels of IL-4 in BALF of Jiaomu Oil and dexamethasone groups,(15.35±4.28) ng/L and (19.20±3.78) ng/L,were both significantly lower than those of asthmatic model group(P0.05).But the levels of IFN-γ in the above two groups,BALF,(50.65±4.19) ng/L,(53.34±5.64) ng/L and PC_(20) (0.160±0.180) g/L,(0.144±0.154) g/L,were significantly higher than those of asthmatic model group (P0.05).The levels of IL-4,IFN-γ and PC_(20) did not have any statistical significance between Jiaomu Oil and dexamethasone groups(P 0.05).The percents of NF-κB positive cells in normal group,asthmatic model group,Jiaomu Oil group and dexamethasone group were (9.51±2.82) %,(52.71±7.80) %,(32.26±(3.70) % and (29.89±2.01) % respectively.The percents of two treatment groups both had statistical significance compared with that of asthmatic model group. Conclusions Jiaomu Oil can efficiently decrease the levels of IL-4 and increase IFN-γ in BALF,it can decrease airway hyperresponsiveress and expression of NF-κB in the asthmatic guinea pig.