Study on the inhibitory effect on coxsackievirus B_3 replication in vitro by the compounded Qiangqi extract

Objective:The compounded Qiangqi extract was used to investigate its antiviral activity in Vero cells as a specific inhibitor of Coxsackievirus B 3 (CVB 3) replication, and its antiviral mechanism was exploredMethods:Vero cells infected by CVB 3 were cultured with different concentration of the compounded Qiangqi extract for 48 hours, and its inhibitory effect on CVB 3 replication was evaluated with cell survival rates by MTT assay and 50% tissue culture infection dose (TCID 50 ) of culture supernatantResults: The compounded Qiangqi extract could significantly inhibit cytopathic effect (CPE) of CVB 3-infected Vero cells, increase survival rates of CVB 3infected cells in dose dependent manner and decrease viral titres of culture supernatantConclusion:It may be the antiviral mechanism of the compounded Qiangqi extract that it may kill viruses directly and inhibit the replication of CVB 3 in cells, without preventing from viral adsorption and penetration