Effects of ADFMChR on the Proliferative Viability of Human Lung Cancer A549 Cell Line

Objective To investigate the effects of 5-allyl-7-gen-difluoromethyllenechrysin(ADFMChR) on the proliferative viability of cultured human lung cancer cell line(A549) and Chinese hamster lung epithelial cell(CHL) in vitro.Methods A549 cell line and CHL cell line were cultured in vitro.The viability inhibition of two cell line were examined by MTT assay.Results The MTT assay showed that the proliferation inhibitory rates of A549 cell line were 24.39%,47.66%,62.75%,73.35%,85.94% respectively after treatment with 1.0,2.0,4.0,8.0,16.0μmol/L of ADFMChR for 48h.ADFMChR significantly suppressed proliferation of A549 cell line in a concentration dependent manner.The efficacy of inhibition with ADFMChR was stronger than that of Chrysin while it was similar to DDP.For CHL cell line,the proliferation inhibitory rates were 3.95%,7.44%,13.89%,21.60%,27.43% respectively after CHL cell was treated with 1.0,2.0,4.0,8.0,16.0 μmol/L of ADFMChR for 48 h.The cytotoxicity of ADFMChR to CHL cells was lower than that of DDP while it was similar to Chrysin.Conclusions ADFMChR had stronger cell proliferation inhibition effect on A549 cell line than to CHL cell line and it possessed the relative selectivity for cell proliferation inhibition on A549 cell.