Induction of apoptosis of human gastric carcinoma SGC-7901 cell line by 8-bromo-7-methoxychrysin

Aim To investigate the effect of 8-bromo-7-methoxychrysin(BrMChR) on apoptosis of human gastric carcinoma SGC-7901 cell line.Methods SGC-7901 cells were cultured and the inhibitory effect of BrMChR on the proliferation of SGC-7901 cell line was measured by MTT assay.The apoptosis of SGC-7901 cells induced by BrMChR was analyzed using flow cytometry (FCM) with PI staining.DNA ladder bands were observed by DNA agarose gel electrophoresis.Results MTT assay suggested that BrMChR markedly inhibited the proliferation of SGC-7901 cells in a concentration-dependent manner,its IC50 was 2.6 μmol·L-1,the potency of BrMChR was 8 times more than that of lead compound,chrysin (ChR,IC50 was 16.5 μmol·L-1),and was 3 times more than that of 5-fluorouracil (5-FU,IC50 was 7.7 μmol·L-1). FCM with PI staining indicated the apoptotic rate of SGC-7901 cell line treated with BrMChR (1.25,5.00,20.00 μmol·L-1) for 48 h was (19.8%±0.2%),(36.8%±1.9%) and (45.5%±3.5%) respectively and the rate treated with BrMChR (1.25 μmol·L-1) was higher than that treated with ChR (20.00 μmol·L-1,12.9%±1.5%). DNA agarose gel electrophoresis showed that when treated with BrMChR(20.00 μmol·L-1) for 48 h,the gene DNA of SGC-7901 cells presented typical DNA ladder bands which could attenuated by the effect of pre-incubation of GW9662(10.00 μmol·L-1),a blocker of PPARγ.Conclusion BrMChR might induce apoptosis of SGC-7901 cell line partially by activating PPARγ.