Experimental Study on Repairing of Skin Defection with Tissue-engineered Skin

Objective To investigate the feasibility of repairing animal skin defection with tissue-engineered skin made by cultured keratinocytes and acellular dermal matrix.Methods Tissue-engineered skin,which was constructed with cultured human keratinocytes and acellular dermal matrix,was transplanted to full skin wound in athymic mice.The wounds were oberserved after operation.Tissue samples were harvested and exminded by means of histology and immunohistochemistry.Results The full-thickness skin wounds in athymic mice repaired with tissue-engineered skin were healing well.The newly grown epithelium was demonstrated originated from human keratinocytes.Conclusion It is feasible to repair full-thickness wounds with tissue-engineered skin made by cultured keratinocytes and acellular dermal matrix.